The used 1H NMR for Screening on Streptomyces spp. and Identification of Metabolites Involved in Both Antifungal and Non-antifungal Producer for Colletotrichum gleosporioides
DOI:
https://doi.org/10.53797/agrotech.v3i1.6.2024Keywords:
Metabolites, Antifungal activity, Streptomyces spp., Colletotrichum gleosporioides, Nuclear Magnetic ResonaceAbstract
Streptomyces spp. had been well known for their antimicrobial activities. These activities were due to the presence of certain metabolites in the Streptomyces spp. In this study, Streptomyces spp. with the ability to produce antimicrobial activity and those without activity were grown in Starch Casein Broth (SCB) for 3 days before the metabolites were extracted using the liquid-liquid partition method. The obtained extracts were then subjected to 1H Nuclear Magnetic Resonance (NMR) analysis to obtain the metabolites presence in both antifungal producing and non-antifungal producing Streptomyces spp. From the analysis, it was observed that valine, isoleucine, leucine, asparagine, α-glucose, and fructose were present in antifungal producing Streptomyces spp., while only fatty acid and lactic acid were observed for non-antifungal producing Streptomyces spp. The use of 1H NMR does not only help in identifying Streptomyces spp. with potential antifungal ability but can also help in identifying the Streptomyces spp.
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