Isolation and Screening of Bioactivities from Bacillus sp. Isolated from Banana Rhizosphere
DOI:
https://doi.org/10.53797/agrotech.v2i2.8.2023Keywords:
Bacillus sp., secondary metabolites, Malaysia soil, seed germination test, plant growth promoterAbstract
Bacillus sp. has been widely known to produce various bioactive metabolites related to plant growth. Bacillus sp. has also been known as one of the plant growth-promoting bacteria. In this study, a total of 68 isolates of Bacillus sp. were isolated from the rhizosphere of banana plantation using soil suspension technique. Isolated Bacillus sp. was then subjected to screening of their secondary metabolites such as cellulase, mannanase, xylanase, indole-acetic-acid, and gibberellic acid production before potential isolates were chosen and identified using their 16S rRNA and later subjected to seed germination testing. It was observed that Bacillus sp. isolated produces most of cellulase but did not have much affinity to produce gibberellic acid as only 5 Bacillus sp. were observed producing gibberellic acid. Three of the potential Bacillus sp. were selected and identified as Bacillus pumilus strain PNO21, Bacillus amyloliquefaciens strain PNO7 and Bacillus wiedmannii strain PNO28. Seed germination test also revealed that these 3 Bacillus sp. did not inhibit the seeds germination.
Downloads
References
Balla, A., Silini, A., Cherif-Silini, H., Bouket, A.C., Boudechicha, A., Luptakova, L., Alenezi, F.N. & Belbahri, L. (2022). Screening of cellulolytic bacteria from various ecosystems and their cellulases production under multi-stress conditions. Catalysts, 12, 769. https://doi.org/10.3390/catal12070769
Cabra Cendales, T., Rodríguez González, C.A., Villota Cuásquer, C.P., Tapasco Alzate, O.A. & Hernández Rodríguez, A. (2017). Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L). Acta Biologica Colombiana, 22(1), 37-44. http://dx.doi.org/10.15446/abc.v22n1.57375
Caulier, S., Nannan, C., Gillis, A., Licciardi, F., Bragard, C. & Mahillon, J. (2019). Overview of the antimicrobial compounds produced by members of the Bacillus subtilis group. Frontiers of Microbiology, 10, 302. https://doi.org/10.3389/fmicb.2019.00302.
Dobrzyński, J., Wróbel, B. & Górska, E.B. (2022). Cellulolytic Properties of a Potentially Lignocellulose-Degrading Bacillus sp. 8E1A Strain Isolated from Bulk Soil. Agronomy, 12, 665. https://doi.org/10.3390/agronomy12030665
Fan, B., Blom, J., Klenk, H.P. & Borriss, R. (2017). Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis form an “operational group B. amyloliquefaciens” within the B. subtilis species complex. Frontiers of Microbiology, 8, 22. https://doi.org/10.3389/fmicb.2017.00022.
Harwood, C.R., Mouillon, J.M., Pohl, S. & Arnau, J. (2018). Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group. FEMS Microbiology Reviews, 42, 721–738. https://doi.org/10.1093/femsre/fuy028.
Hsiao, C.Y., Blanco, S.D., Peng, A.L., Fu, J.Y., Chen, B.W., Luo, M.C., Xie, X.Y. & Lin, Y.H. (2023). Seed treatment with calcium carbonate containing Bacillus amyloliquefaciens PMB05 powder is an efficient way to control black rot disease of cabbage. Agriculture, 13(5), 926. http://dx.doi.org/10.3390/agriculture13050926.
Iqbal, A. & Hasnain, S. (2013). Auxin producing Pseudomonas strains: Biological candidates to modulate the growth of Triticum aestivum beneficially. American Journal of Plant Sciences, 4, 1693-1700.1.1
Jeffrey, L.S.H., Norzaimawati, A.N. & Halizah, H. (2011). Prescreening of bioactivities from actinomycetes isolated from forest peat soil of Sarawak. Journal of Tropical Agriculture and Food Science, 30(2), 245-254.
Kaspar, F., Neubauer, P. & Gimpel, M. (2019). Bioactive secondary metabolites from Bacillus subtilis: A comprehensive review. Journal of Natural Products, 82, 2038–2053. https://doi.org/10.1021/ acs.jnatprod.9b00110.
Kovács, Á.T. (2019). Bacillus subtilis. Trends in Microbiology, 27, 724–725. https://doi.org/10.1016/j.tim.2019.03.008.
Manzum, A.A. & Al Mamun, M.A. (2018). Isolation of Bacillus from soil for production of celllulase. Nepal Journal of Biotechnology, 6(1), 57-61. https://doi.org/10.3126/njb.v6i1.22338
Miljakovi’c, D., Marinkovi´c, J. & Baleševi´c-Tubi´c, S. (2020). The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms, 8, 1037; http://doi.org/10.3390/microorganisms8071037.
Ongena, M. & Jacques, P. (2008). Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology, 16, 115–125. https://doi.org/10.1016/j.tim.2007.12.009.
Pandya, N.D, & Desai, P.V. (2014). Screening and characterization of GA3 producing Pseudomonas monteilii and its impact on plant growth promotion. International Journal of Current Microbiology and Applied Sciences, 3(5), 110-115.
Suliasih & Widawati, S. (2020). Isolation of Indole Acetic Acid (IAA) producing Bacillus siamensis from peat and optimization of the culture conditions for maximum IAA production. IOP conference Series: Earth and Environmental Science, 572. https://doi:10.1088/1755-1315/572/1/012025.
Stein, T. (2005). Bacillus subtilis antibiotics: structures, syntheses and specific functions. Molecular Microbiology, 56, 845–857. https://doi.org/10.1111/j.1365-2958.2005.04587.x.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 arsvot
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
