Effect of Varying NAA Hormone Concentrations on In Vitro Subculture of Raja Bulu Banana (Musa sp.)
DOI:
https://doi.org/10.53797/agrotech.v4i1.9.2025Keywords:
NAA, in vitro rooting, Musa sp., tissue culture, root inductionAbstract
Tissue culture, a method involving the sterilized in vitro propagation of plant parts, offers potential for producing disease-free seedlings. This study aimed to (1) evaluate the effect of NAA (1-naphthaleneacetic acid) concentrations on root induction in Raja Bulu banana (Musa sp.) plantlets; (2) assess rooting percentage, root count, leaf number, root length, root mass, leaf color, plant height, and stem diameter; and (3) identify the optimal NAA concentration. Conducted from January to March 2025 at Salaman, Magelang (270 m a.s.l.), the experiment employed a Completely Randomized Design (CRD) with four NAA treatments (0, 2, 4, and 6 ppm) and six replications. Each bottle culture contained two plantlets. After 35 days, plantlets treated with 2 ppm NAA exhibited the highest root count (5.83 roots), with superior performance across other parameters. Elevated NAA concentrations (4–6 ppm) resulted in decreased rooting and chlorophyll content, indicating potential hormonal imbalance and phytotoxicity. The study concludes that 2 ppm NAA is the most effective concentration for rooting Raja Bulu banana in vitro. Future research should investigate the combined effects of auxins and cytokinins, natural biostimulants, and varying culture conditions to further enhance propagation efficiency.
Downloads
References
Ali, S., Shah, M. A., Ahmad, N., & Khan, M. R. (2021). Pengaruh berbagai konsentrasi auksin terhadap perakaran in vitro kultivar zaitun Moraiolo. Jurnal Ilmu dan Penelitian Pertanian, 8(2), 23–29. https://doi.org/10.31227/osf.io/k9qhs
Aprinda, O., Lizawati, & Eliyanti. (2022). Induksi akar pada eksplan tunas anggrek (Dendrobium var. Airy Beauty) secara in vitro dengan penambahan naphtalene acetic acid (NAA) dan 6-benzyl amino purin (BAP). Jurnal Agroecotenia, 5(1), 27–39. https://doi.org/10.32502/jae.v5i1.5105
Debitama, A. M. N. H., Iga, A. M., & Ummul, H. (2022). Pengaruh hormon auksin sebagai zat pengatur tumbuh pada beberapa jenis tumbuhan Monocotyledoneae dan Dicotyledoneae. Jurnal Biologi dan Pembelajarannya, 17(1), 120–130. https://doi.org/10.17977/um043v17i1p120-130
Erisa, R., Steffanie, N., Dedi, S. R. R., & Sri Astuti, M. (2022). Pengaruh konsentrasi 6-benzyl amino purine (BAP) dan media Murashige dan Skoog (MS) terhadap pertumbuhan dan perkembangan subkultur anggrek Dendrobium sp. Wooleng secara in vitro. Seminar Nasional Pendidikan Biologi dan Saintek (SNPBS), 7, 83–93.
Fadhila, D., Siti, H., & Wiwin, T. I. (2023). Kerapatan stomata, warna, dan kadar klorofil daun kelakai (Stenochlaena palustris (Burm. F) Beddome) berdasarkan perbedaan lokasi tumbuh dan tingkat umur daun. Journal of Forest Science Avicenina, 6(1), 78–84. https://doi.org/10.37275/jfsa.v6i1.338
Hartati, S., Agus, B., & Ongko, C. (2016). Pengaruh NAA dan BAP terhadap pertumbuhan subkultur anggrek hasil persilangan Dendrobium biggibum × Dendrobium liniale. Journal of Sustainable Agriculture, 31(1), 33–37. https://doi.org/10.24198/jsa.v31i1.11462
Hasidah, Mukarlina, & Diah, W. R. (2017). Kandungan pigmen klorofil, karotenoid dan antosianin daun Caladium. Jurnal Protobiont, 6(2), 29–36. https://doi.org/10.30872/protobiont.v6i2.1500
Jannah, K. P. A., Prihantoro, I., & Karti, P. D. M. H. (2023). Optimasi level benzyl amino purin (BAP) terhadap pertumbuhan tanaman kembang telang (Clitoria ternatea) melalui teknik kultur jaringan. Jurnal Ilmu Nutrisi dan Teknologi Pakan, 21(2), 100–106. https://doi.org/10.25047/jintp.v21i2.3059
Khozin, M. N., Restanto, D. P., & Kusbianto, D. E. (2022). Embriogenesis somatik langsung dan tidak pada tanaman porang (Amorphophallus oncophyllus). Jurnal Agrotek Indonesia, 7(2), 42–45. https://doi.org/10.29239/j.agrin.v7i2.3717
Maninggolang, A., Jeany Sh, P.-M., & Wenny, T. (2018). Pengaruh BAP (benzyl amino purine) dan air kelapa terhadap pertumbuhan tunas pucuk dan kandungan sulforafan brokoli (Brassica oleracea L. var. italica Plenck) secara in vitro. Agri-Sosio Ekonomi Unsrat, 14(1), 585–596. https://ejournal.unsrat.ac.id/index.php/jisep/article/view/22353
Mubarak, M. Z., & Evie, R. (2024). Multiplikasi planlet Musa acuminata C. dengan penambahan NAA dan air kelapa secara in vitro. LenteraBio, 13(2), 205–211. https://doi.org/10.15294/lentera.v13i2.56789
Nigrum, W. C., Rahmad, J., & Wiharyanti, N. L. (2024). Pengaruh pemberian NAA dan kinetin terhadap pertumbuhan eksplan pisang Cavendish (Musa paradisiaca L.) melalui teknik kultur jaringan secara in vitro. Jurnal Tropicrops, 7(1), 11–23. https://doi.org/10.32734/jtr.v7i1.10123
Pratama, R. A., Yunira, R., Noertjahyani, K., Kelik, P., & Raden, H. (2022). Pengaruh napthalane acetic acid dan benzyl amino purine terhadap mikropropagasi tanaman akar wangi (Vetiveria zizanioides L. Nash). Jurnal Penelitian Pertanian, 5(2), 50–56. https://doi.org/10.22146/jpp.2022.23103
Putri, R. R. D., Suwirmen, & Nasril, N. (2018). Pengaruh napthalene asam asetat (NAA) pada pertumbuhan akar pisang raja Kinalun secara in vitro. Jurnal Biologi Universitas Andalas, 6(1), 1–5. https://doi.org/10.25077/jbua.v6i1.1022
Saktiyono, S. T. P. (2015). Pengaruh konsentrasi NAA dan BAP terhadap pertumbuhan tunas eksplan tanaman pisang Cavendish (Musa paradisiaca L.) melalui kultur in vitro. Gontor Agrotech Science Journal, 2(1), 31–37. https://doi.org/10.21111/gasj.v2i1.334
Tika, Y. Y., & Sudarti, S. (2021). Pengaruh intensitas cahaya terhadap pertumbuhan tanaman kunyit. Jurnal Penelitian Fisika dan Terapannya (JUPITER), 2(2), 52–57. https://doi.org/10.25008/jupiter.v2i2.2435
Ziadaturrif’ah, D., Sri, D., & Rini, B. (2019). Potensi autoalelopati ekstrak daun kirinyuh (Chromolaena odorata L.). Buletin Anatomi dan Fisiologi, 4(2), 129–136. https://doi.org/10.25047/baf.v4i2.1122
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 arsvot
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
