Molecular Identification of Planococcus citri (Hemiptera: Pseudococcidae)
DOI:
https://doi.org/10.53797/agrotech.v2i2.4.2023Keywords:
Planococcus citri, COI, 18S rDNA gene, ITS2 gene, USA, IsraelAbstract
Planococcus citri is an important phytophagous pest in different agroecosystems. The species is very difficult to distinguish by morphological features. The goal of this research was to present basic data on the molecular data to confirm P. citri. The primers used in the molecular identification of P. citri consisted of mitochondrial cytochrome c oxidase subunit 1 (COI), ribosomal small subunit RNA (18S rDNA) gene, and internal transcribed spacer 2 (ITS2) gene. The 1% (w/v) agarose gel showed the presence of successful amplification of the COI, 18S rDNA, and ITS2 genes indicated with positive PCR products. The Internal transcribed spacer (ITS2), 18S ribosomal and Cytochrome c oxidase I (COI) mitochondrial genes used in this study confirmed the identity of mealybugs as P. citri. The 18S ribosomal gene showed 98% nucleotide sequence homology to the P. citri USA isolate while the COI gene and ITS2 gene showed less than 95% nucleotide sequence homology to the P. citri USA isolate and P. citri Israel isolate, respectively. At present, the nucleotide sequence data of mealybugs is still lacking; consequently, mealybug species must be correctly identified for the proper management of this pest.
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